baby BotryllusComplete publication list as PDF
Tunicate Involved Publications:


Corey, Daniel M., Benyamin Rosental, Mark Kowarsky, Rahul Sinha, Katherine J. Ishizuka, Karla J. Palmeri, Stephen R. Quake, Ayelet Voskoboynik, and Irving L. Weissman. "Developmental cell death programs license cytotoxic cells to eliminate histocompatible partners." Proceedings of the National Academy of Sciences (2016): 201606276.

Gasparini, Fabio, Tatjana Skobo, Francesca Benato, Giorgia Gioacchini, Ayelet Voskoboynik, Oliana Carnevali, Lucia Manni, and Luisa Dalla Valle. "Characterization of Ambra1 in asexual cycle of a non-vertebrate chordate, the colonial tunicate Botryllus schlosseri, and phylogenetic analysis of the protein group in Bilateria." Molecular phylogenetics and evolution 95 (2016): 46-57.

Stolfi, A., Sasakura, Y., Chalopin, D., Satou, Y., Christiaen, L., Dantec, C., Endo, T., Naville, M., Nishida, H., Swalla, B. J., Volff, J.-N., Voskoboynik, A., Dauga, D. and Lemaire, P. (2015), Guidelines for the nomenclature of genetic elements in tunicate genomes. genesis, 53: 1–14. doi:10.1002/dvg.22822

Voskoboynik, Ayelet, and Irving L. Weissman. "Botryllus schlosseri, an emerging model for the study of aging, stem cells, and mechanisms of regeneration." Invertebrate reproduction & development 59, no. sup1 (2015): 33-38.

Manni, Lucia, Fabio Gasparini, Kohji Hotta, Katherine J. Ishizuka, Lorenzo Ricci, Stefano Tiozzo, Ayelet Voskoboynik, and Delphine Dauga. "Ontology for the asexual development and anatomy of the colonial chordate Botryllus schlosseri." PloS one 9, no. 5 (2014): e96434.

Griggio, Francesca, Ayelet Voskoboynik, Fabio Iannelli, Fabienne Justy, Marie-Ka Tilak, Turon Xavier, Graziano Pesole, Emmanuel JP Douzery, Francesco Mastrototaro, and Carmela Gissi. "Ascidian mitogenomics: comparison of evolutionary rates in closely related taxa provides evidence of ongoing speciation events." Genome biology and evolution 6, no. 3 (2014): 591-605.

Voskoboynik A, Newman AM, Corey DM, Sahoo D, Pushkarev D, Neff NF, Passarelli B, Koh W, Ishizuka KJ, Palmeri KJ, Dimov I, Keasar C, Fan CH, Mantalas GL, Sinha R, Penland L, Quake SR, Weissman IL. Identification of a colonial chordate histocompatibility gene. Science 2013 26;341(6144):384-7. doi: 10.1126/science.1238036. PMID:23888037

Voskoboynik A, Neff NF, Sahoo D, Newman AM, Pushkarev D, Koh W, Passarelli B, Fan CH, Mantalas GL, Palmeri KJ, Ishizuka KJ, Gissi C, Griggio F, Ben-Shlomo R, Corey DM, Penland L, White RAIII, Weissman IL,  Quake SR. The genome sequence of the colonial chordate Botryllus schlosseri. eLIFE 2013. DOI:10.7554/eLIFE.00569

Rinkevich Y, Voskoboynik A, Rosner A, Rabinowitz C, Paz G, Oren M, Douek J, Alfassi G, Moiseeva E, Ishizuka KJ, Palmeri KJ, Weissman IL, Rinkevich B, Repeated, long termcycling of putative stem cells between niches in a casal chordate. Dev Cell 2013 24: 76-88. doi:10.1016/j.devcel.2012.11.010

Voskoboynik A, Rinkevich B, Weissman IL. Stem cells, Chimerism and Tolerance: Lessons from Mammals and Ascidians (review; 30 pages). In: Rinkevich B., and Matranga V. editors. Stem Cells in Marine Organisms. Springer. 2009.

Voskoboynik A, Soen Y, Rinkevich Y, Rosner A. Ueno H, Reshef R, Ishizuka KJ, Palmeri KJ, Moiseeva E, Rinkebich B, Weissman IL. Identification of the endostyle as a stem cell niche in a colonial chordate. Cell Stem Cell. 2008 Oct 9;3(4):456-64.

Voskoboynik A, Simon-Blecher N, Soen Y, Rinkevich B, DeTomaso AW, Ishizuka KJ, Weissman IL. Striving for normality: whole body regeneration through a series of abnormal generations. FASEB J. 2007 May;21(7):1335-44.

Nyholm SV, Passegue E, Ludington WB, Voskoboynik A, Mitchel K. Weissman IL, DeTomaso AW. Fester, A candidate allorecognition receptor from a primitive chordate. Immunity. 2006 Jul;25(1):163-73.
Laird DJ, De Tomaso AW, Weissman IL.  Stem cells are units of natural selection in a colonial ascidian. Cell 2005 Dec 22.

De Tomaso  AW, Nyholm SV, Palmeri  KJ, Ishizuka , KJ, Ludington WB, Mitchel K, Weissman IL.  Isolation and characterization of a protochordate histocompatibility locus.  Nature 2005 November 24; 438: 454-459.
Laird DJ, Weissman IL. Telomerase maintained in self-renewing tissues during serial regeneration of the urochodate Botryllus schlosseri. Dev Biol. 2004 Sep 15;273(2):185-94.

De Tomaso AW, Weissman IL. Evolution of a protochordate allorecognition locus. Science. 2004 Feb 13;303(5660);977.

Laird DJ, Weissman IL. Continuous development precludes radioprotection in a colonial ascidian. Dev Comp Immunol.2004; 28(3): 201-9.

De Tomaso AW, Weissman IL. Construction and characterization of large-insert genomic libraries (BAC and fosmid) from the Ascidian Botryllus schlosseri and initial physical mapping of a histocompatibility locus. Mar Biotechnol (NY). 2003; 5(2): 103-15.

De Tomaso AW, Weissman IL. Initial characterization of a protochordate histocompatibility locus. Immunogenetics. 2003 Oct; 55(7): 480-90.

Stoner DS, Ben-Shlomo R, Rinkevich B, Weissman IL. Genetic variability of Botryllus schlosseri invasions to the east and west coasts of the USA. Marine Ecology Progress Series, 2002: p. 93-100.

Lauzon RJ, Ishizuka KJ, Weissman IL. Cyclical generation and degeneration of organs in a colonial urochordate involves crosstalk between old and new: a model for development and regeneration. Dev Biol. 2002; 249: 333-348.

Lauzon RJ, Rinkevich B, Patton CW, Weissman IL. A morphological study of nonrandom senescence in a colonial urochordate.Biol Bull. 2000; 198: 367–378.

Laird DJ, De Tomaso AW, Cooper MD, Weissman IL. 50 million years of chordate evolution: seeking the origins of adaptive immunity. PNAS. 2000; 97: 6924–6926.

Stoner D, Rinkevich B, Weissman IL. Heritable germ and somatic cell lineage competitions in chimeric colonial protochordates.PNAS. 1999; 96: 9148–9153.

Rinkevich B, Weissman IL, De Tomaso AW. Transplantation of Fu/HC–incompatible zooids in Botryllus schlosseri results in chimerism. Biol Bull. 1998; 195: 98–106.

De Tomaso AW, Saito Y, Ishizuka KJ, Palmeri KJ, Weissman IL. Mapping the genome of a model protochordate. I. A low resolution genetic map encompassing the fusion/histocompatibility (Fu/HC) locus of Botryllus schlosseri. Genetics. 1998; 149:277-287.

Stoner DS, Quattro JM, Weissman IL. Highly polymorphic microsatellite loci in the colonial ascidian Botryllus schlosseri. Mol Marine Biol Biotech. 1997; 6: 163–171.

Stoner DS, Weissman IL. Somatic and germ cell parasitism in a colonial ascidian: possible role for a highly polymorphic allorecognition system. PNAS. 1996; 93: 15254–15259.

Rinkevich B, Weissman IL, Shapira M. Alloimmune hierarchies and stress–induced reversals in the resorption of chimeric protochordate colonies. Proc R Soc Lond. B 1994; 258: 215–220.

Rinkevich B, Saito Y, Weissman IL. A colonial invertebrate species that displays a hierarchy of allorecognition responses. Biol Bull 1993; 184: 79–86.

Lauzon R, Patton C, Weissman IL. A morphological and immunohistochemical study of programmed cell death in Botryllusschlosseri. Cell and Tissue Res. 1993; 272: 115–127.

Lauzon R, Ishizuka K, Weissman IL. A cyclical, developmentally–regulated death phenomenon in a colonial urochordate.Developmental Dynamics. 1992; 194: 71–83.

Rinkevich B, Lauzon RJ, Brown BWM, Weissman IL. Evidence for a programmed life span in a colonial protochordate. PNAS.1992; 89: 3546–3550.

Rinkevich B, Weissman IL. Chimeras vs genetically homogenous individuals: potential fitness costs and benefits. Oikos. 1992; 63: 119–124.

Rinkevich B, Weissman IL. Allogeneic resorption in colonial protochordates: consequences of nonself recognition. Develop and Comp Immunol. 1992; 16: 275–286.

Rinkevich B, Weissman IL. Incidents of rejection and indifference in Fu/HC incompatible protochordate colonies. J Exp Zool.1992; 263: 105–111.

Rinkevich B, Shapira M, Weissman IL, Saito Y. Allogeneic responses between three remote populations of the cosmopolitan ascidian Botryllus schlosseri. Zool Sci. 1992; 9: 989–994.

Rinkevich B, Weissman IL. Interpopulational allogeneic reactions in the colonial protochordate Botryllus schlosseri. Intl Immunol. 1991; 3: 1265–1272.

Rinkevich B, Weissman IL. Failure to find alloimmune memory in the resorption phenomenon of Botryllus cytomictical chimera.Eur J Immunol. 1990; 20: 1775–1779.

Lauzon R, Weissman IL. The paternal centrosome directs the polarity of early pattern formation in the fertilized ascidia ceratodes egg. In: Hoshi M, Yamashita O, eds. Advances in Invertebrate Reproduction 5. Amsterdam: Elsevier/North Holland Biomedical Press; 1990: 131–138.

Boyd HC, Weissman IL, Saito Y. Morphologic and genetic verification that Monterey Botryllus and Woods Hole Botryllus are the same species. Biol Bull. 1990; 178: 239–250.

Rinkevich B, Weissman IL. Botryllus schlosseri (Tunicata) whole colony irradiation: do senescent zooid resorption and immunological resorption involve similar recognition events? J Exp Zool. 1990; 253: 189–201.

Rinkevich B, Weissman IL. Variation in the outcomes following chimera formation in the colonial tunicate Botryllus schlosseri. Bull Marine Sci. 1989; 45: 213–227.

Weissman IL, SaitoY, Rinkevich B. Allorecognition in colonial tunicates; parallels with and tangents from vertebrate immunity. In: Melchers F, ed. Progress in Immunology. Vol. 7. Berlin: Springer–Verlag; 1989: 1256–1263.

Rinkevich B, Weissman IL. Retreat growth in the ascidian Botryllus schlosseri: a consequence of nonself recognition. In: Grosberg R, Hedgecock D, Nelson K, eds. Invertebrate Historecognition. New York: Plenum Publishing Corporation; 1988: 93–109.

Weissman IL, Scofield V, Saito Y, Boyd H, Rinkevich B. Speculations on the relationships of two Botryllus allorecognition reactions —colony specificity and resorption —to vertebrate histocompatability. In: Grosberg R, Hedgecock D, Nelson K, eds.Invertebrate Historecognition. New York: Plenum Publishing Corporation; 1988; 67–78.

Rinkevich B, Weissman IL. The fate of Botryllus (Ascidiacea) larvae cosettled with parental colonies: beneficial or deleterious consequences? Biol Bull. 1987; 173: 474–488.

Rinkevich B, Weissman IL. Chimeras in colonial invertebrates: a synergistic symbiosis or somatic– and germ–cell parasitism?Symbiosis. 1987; 4: 117–134.

Rinkevich B, Weissman IL. A long–term study on fused subclones in the ascidian Botryllus schlosseri: the resorption phenomenon (Protochordata: tunicate). J Zoology. 1987; 213: 717–733.

Boyd HC, Brown SK, Harp JA, Weissman, IL. Growth and sexual maturation of laboratory–cultured Monterey Botryllus schlosseri. Biol Bull. 1986; 170: 91–109.

Schlumpberger J, Weissman IL, Scofield VL. Separation and labeling of specific subpopulations of Botryllus blood cells. J Exp Zool. 1984; 229: 401–411.

Schlumpberger J, Weissman IL, Scofield VL. Monoclonal antibodies developed against Botryllus blood cell antigens bind to cells of distinct lineages during embryonic development. J Exp Zool. 1984; 229: 205–213.

Scofield VL, Schlumpberger JM, Weissman IL. Colony specificity in the colonial tunicate Botryllus and the origins of vertebrate immunity. Am Zoologist. 1982; 22: 783–794.

Scofield VL, Schlumpberger JM, West LA, Weissman IL. Protochordate allorecognition is controlled by an MHC–like gene system. Nature. 1982; 295: 499–502.

Weissman IL, Scofield VL, Schlumpberger J, Pillemer E, Hollander N, Rouse RV, Stevens SK, Butcher EC. Functional considerations of normal and neoplastic T lymphocytes. Presented at the Symposium on “The Biological Significance to Immune Regulation”, Seattle, Washington, December 27–31, 1980. In: Ruben LN, Gershwin ME, eds. Immune Regulation, Evolutionary and Biological Significance. New York: Marcel Dekker; 1982: 99–113.

Scofield VL, Weissman IL. Allorecognition in biological systems. Devel and Comp Immunol. 1981; 5: 23–28.